I have broad background in immune response-related molecular and cellular biology with our animal models and have been leading and designing research projects throughout my research career. My work focused on investigating the biological functions of dietary plant nanoparticles & cellular organelles on macrophage/microglia contributing to inflammation-mediated diseases including neurodegeneration and tumor progression. I performed numerous efficacy studies of plant-derived nanoparticles modulating macrophage/microglia in the innate immune response, such as ginger-derived exosomal microRNAs (miRNA) mitigate colitis by modulating the metabolism of gut probiotics (2018) and COVID-19-related lung inflammation by repressing the expression of viral protein NSPs (2021). Most recently, I have extensive experience in central nervous system (CNS) inflammation and demonstrated that gut bacterial metabolites such as isoamylamine (IAA) become more prevalent in the aged gut due to the presence of more Ruminococcaceae. Using the single-strand gel shift (SSGS) innovative technique that I originally developed, I demonstrated an epigenetic regulatory mechanism in aging that the gut bacterial metabolite IAA promotes microglia-related brain inflammation and cognitive decline in aging by directly targeting proinflammatory genes in microglia. Targeting and modulating microglia-related neuroinflammation would be therapeutic potential for neurodegeneration caused by various causes such as aging, diabetes and alcohol. Using this technique, we defined that necessary amino acid such as tryptophan not only participate protein but also exerts a novel role of modulating miRNA activating by targeting Argonaute protein and assembling in RNA-induced silencing complex (RISC) (2024). My laboratory has expertise in investigating how broad-derived extracellular nanoparticles and cellular organelles are used as nanovesicles to deliver therapeutic agents in neurodegeneration and cancer treatment. Most notably, for the past 5 years my group has advanced our understanding of the roles that dietary plants play in macrophage/microglia-mediated innate immunity. The scientific work proposed in this application focuses on edible plant-derived mitochondria in the communication of the gut-brain axis modulating brain function, which is the potential of mitochondrial transfer-based therapy and uninvestigated yet.